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KMID : 0360319970290020266
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Journal of Korean Cancer Research Association
1997 Volume.29 No. 2 p.266 ~ p.279
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Prognostic Significance of Histologic Features, DNA Content, Expression of Proliferating Cell Nuclear Antigen (PCNA), c-fos Protein and Transforming Growth Factor(TGF)-a and -βin Giant Cell Tumor of Bone
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Abstract
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Purpose:
@EN This study was attempted to investigate the prevalence of the expression of c-fos protein, TGF-αand -β, PCNA , DNA ploidy pattern and histopathological parameters of giant cell tumor (GCT) of bone and to correlate with prognosis and to
extend
our
understanding on tumorigenesis of GCT.
@ES Materials and Methods:
@EN Twenty eight cases of paraffin-embedded tissue were studied, classified as recurrent (5 cases) and non-recurrent group (12 cases) within the limits of the cases which afforded surgical material on first operation.
@ES Results:
@EN No significant difference was observed in cellularity of stromal cells, atypia of stromal and giant cells, presence of hemorrhage and necrosis between recurrent and non-recurrent group. However, presence of more than 10 mitotic figures in 10
high
power fields in recurrent group was significantly higher than non-recurrent group (P<0.05). The immunoreactivity for PCNA was seen only in nuclei of stromal cells, whereas nuclei of giant cells showed negative staining. The positivity of PCNA
revealed
no significant difference between non-recurrent (mean; 40.9%) and recurrent group (34.4%). The expression of c-fos oncogene was seen in 5 cases (100%) in recurrent group, and 8 cases (66.7%) in non-recurrent group, and no significant difference
was
seen. No significant difference of expression of TGF-a was seen in 5 cases (100%) in recurrent group and in 11 cases (91.7%) in non-recurrent group. The expression of TGF-β in stromal cells was significantly higher in non-recurrent group (80%)
compared
to recurrent group (100%) (P<0.05). In DNA analysis out of 18 cases, 4 cases (22.2%) were aneuploidy and 14 cases (77.8%) were diploidy. Among 4 aneuploidy cases, 3 cases (75%) had no recurrence, and 1 case (25%) had metastasis to lung and
expired.
No
significant difference of DNA ploidy pattern was seen between the recurrent and non-recurrent group.
@ES Conclusion:
@EN Presence of more than 10 mitotic figures in 10 high power fields and less expression of TGF-β are related to higher possibility of recurrence and it is suggested that the number of mitotic figure (more than 10/10HPF) and expression of
TGF-βcould
be helpful parameters in predicting recurrence of GCT.
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KEYWORD
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